1TQQ

Structure of TolC in complex with hexamminecobalt

Summary for 1TQQ

• Entry DOI: 10.2210/pdb1tqq/pdb
• Descriptor: Outer membrane protein tolC, COBALT HEXAMMINE(III) (3 entities in total)
• Functional Keywords: beta-barrel, alpha-barrel, transport protein
• Biological source: Escherichia coli
• Cellular location: Cell outer membrane; Multi-pass membrane protein: P02930
• Total number of polymer chains: 3
• Total formula weight: 154689.86

Authors

Higgins, M.K.,Eswaran, J.,Edwards, P.C.,Schertler, G.F.,Hughes, C.,Koronakis, V. (deposition date: 2004-06-18, release date: 2004-07-20, Last modification date: 2023-08-23)

Primary citation

Higgins, M.K.,Eswaran, J.,Edwards, P.C.,Schertler, G.F.,Hughes, C.,Koronakis, V.
Structure of the ligand-blocked periplasmic entrance of the bacterial multidrug effllux protein TolC
J.Mol.Biol., 342:697-702, 2004

PubMed Abstract: The trimeric TolC protein of Escherichia coli comprises an outer membrane beta-barrel and a contiguous alpha-helical barrel projecting across the periplasm. This provides a single 140 A long pore for multidrug efflux and protein export. We have previously reported that trivalent cations such as hexammine cobalt can severely inhibit the conductivity of the TolC pore reconstituted in planar lipid bilayers. Here, isothermal calorimetry shows that Co(NH(3))(6)(3+) binds to TolC with an affinity of 20 nM. The crystal structure of the TolC-Co(NH(3))(6)(3+) complex was determined to 2.75 A resolution, and showed no significant difference in the protein when compared with unliganded TolC. An electron density difference map revealed that a single ligand molecule binds at the centre of the periplasmic entrance, the sole constriction of TolC. The octahedral symmetry of the ligand and the three-fold rotational symmetry of the TolC entrance determine a binding site in which the ligand forms hydrogen bonds with the Asp(374) residue of each monomer. When Asp(374) was substituted by alanine, high affinity ligand binding was abolished and inhibition of TolC pore conductivity in lipid bilayers was alleviated. Comparable effects followed independent substitution of the neighbouring Asp(371), indicating that this aspartate ring also contributes to the high affinity ligand binding site. As the electronegative entrance is widely conserved in the TolC family, it may be a useful target for the development of inhibitors against multidrug resistant pathogenic bacteria.

PubMed: 15342230
DOI: 10.1016/j.jmb.2004.07.088

Experimental method

X-RAY DIFFRACTION (2.75 Å)