1TOA

PERIPLASMIC ZINC BINDING PROTEIN TROA FROM TREPONEMA PALLIDUM

1TOA の概要

• エントリーDOI: 10.2210/pdb1toa/pdb
• 分子名称: PROTEIN (PERIPLASMIC BINDING PROTEIN TROA), ZINC ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: periplasmic binding protein, zinc binding protein, abc transporter, binding protein
• 由来する生物種: Treponema pallidum
• 細胞内の位置: Periplasm: P96116
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69246.77

構造登録者

Lee, Y.H.,Deka, R.K.,Norgard, M.V.,Radolf, J.D.,Hasemann, C.A. (登録日: 1999-03-03, 公開日: 1999-07-05, 最終更新日: 2023-12-27)

主引用文献

Lee, Y.H.,Deka, R.K.,Norgard, M.V.,Radolf, J.D.,Hasemann, C.A.
Treponema pallidum TroA is a periplasmic zinc-binding protein with a helical backbone.
Nat.Struct.Biol., 6:628-633, 1999

PubMed Abstract: The crystal structure of recombinant TroA, a zinc-binding protein component of an ATP-binding cassette transport system in Treponema pallidum, was determined at a resolution of 1.8 A. The organization of the protein is largely similar to other periplasmic ligand-binding proteins (PLBP), in that two independent globular domains interact with each other to create a zinc-binding cleft between them. The structure has one bound zinc pentavalently coordinated to residues from both domains. Unlike previous PLBP structures that have an interdomain hinge composed of beta-strands, the N- and C-domains of TroA are linked by a single long backbone helix. This unique backbone helical conformation was possibly adopted to limit the hinge motion associated with ligand exchange.

PubMed: 10404217
DOI: 10.1038/10677

実験手法

X-RAY DIFFRACTION (1.8 Å)