1TKS

Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans

1TKS の概要

• エントリーDOI: 10.2210/pdb1tks/pdb
• 関連するPDBエントリー: 1TKU
• 分子名称: 3,4-dihydroxy-2-butanone 4-phosphate synthase (2 entities in total)
• 機能のキーワード: candida albicans, riboflavin biosynthesis, 3, 4-dihydroxy-2-butanone 4-phosphate synthase, crystal structure synthetic gene, isomerase
• 由来する生物種: Candida albicans
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45369.96

構造登録者

Echt, S.,Bauer, S.,Steinbacher, S.,Huber, R.,Bacher, A.,Fischer, M. (登録日: 2004-06-09, 公開日: 2004-09-07, 最終更新日: 2023-08-23)

主引用文献

Echt, S.,Bauer, S.,Steinbacher, S.,Huber, R.,Bacher, A.,Fischer, M.
Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans.
J.Mol.Biol., 341:1085-1096, 2004

PubMed Abstract: A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5 kDa peptide in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity by two chromatographic steps and was shown to catalyze the formation of L-3,4-dihydroxy-2-butanone 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg(-1) min(-1). Hydrodynamic studies indicated a native molecular mass of 41 kDa in line with a homodimer structure. The protein was crystallized in its apoform. Soaking yielded crystals in complex with the substrate ribulose 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7 angstroms, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate synthase of E. coli for molecular replacement. Structural comparison with the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed a hitherto unknown conformation of the essential acidic active-site loop.

PubMed: 15328619
DOI: 10.1016/j.jmb.2004.06.053

実験手法

X-RAY DIFFRACTION (1.6 Å)