1TKI

AUTOINHIBITED SERINE KINASE DOMAIN OF THE GIANT MUSCLE PROTEIN TITIN

Summary for 1TKI

• Entry DOI: 10.2210/pdb1tki/pdb
• Descriptor: TITIN (2 entities in total)
• Functional Keywords: serine kinase, titin, muscle, autoinhibition
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 74337.80

Authors

Mayans, M.O.,Gautel, M.,Wilmanns, M. (deposition date: 1998-05-29, release date: 1999-06-08, Last modification date: 2024-05-22)

Primary citation

Mayans, O.,van der Ven, P.F.,Wilm, M.,Mues, A.,Young, P.,Furst, D.O.,Wilmanns, M.,Gautel, M.
Structural basis for activation of the titin kinase domain during myofibrillogenesis.
Nature, 395:863-869, 1998

PubMed Abstract: The giant muscle protein titin (connectin) is essential in the temporal and spatial control of the assembly of the highly ordered sarcomeres (contractile units) of striated muscle. Here we present the crystal structure of titin's only catalytic domain, an autoregulated serine kinase (titin kinase). The structure shows how the active site is inhibited by a tyrosine of the kinase domain. We describe a dual mechanism of activation of titin kinase that consists of phosphorylation of this tyrosine and binding of calcium/calmodulin to the regulatory tail. The serine kinase domain of titin is the first known non-arginine-aspartate kinase to be activated by phosphorylation. The phosphorylated tyrosine is not located in the activation segment, as in other kinases, but in the P + 1 loop, indicating that this tyrosine is a binding partner of the titin kinase substrate. Titin kinase phosphorylates the muscle protein telethonin in early differentiating myocytes, indicating that this kinase may act in myofibrillogenesis.

PubMed: 9804419
DOI: 10.1038/27603

Experimental method

X-RAY DIFFRACTION (2 Å)