1TJZ

Solution Structure of the Active Site Stem-Loop of the VS Ribozyme

1TJZ の概要

• エントリーDOI: 10.2210/pdb1tjz/pdb
• 分子名称: VS Ribozyme (1 entity in total)
• 機能のキーワード: rna hairpin, rna stem-loop, protonated adenine, ga base pair, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7127.32

構造登録者

Flinders, J.,Dieckmann, T. (登録日: 2004-06-07, 公開日: 2004-09-28, 最終更新日: 2024-05-22)

主引用文献

Flinders, J.,Dieckmann, T.
The solution structure of the VS ribozyme active site loop reveals a dynamic "hot-spot"
J.Mol.Biol., 341:935-949, 2004

PubMed Abstract: The VS ribozyme is the largest ribozyme in its class and is also the least structurally characterized thus far. The current working model of the VS ribozyme locates the active site in stem-loop VI. The solution structure of this active site loop was determined using high resolution NMR spectroscopy. The structure reveals that the ground-state conformation of the active site differs significantly from that determined previously from chemical structure probing and mutational analysis of the ribozyme in its active conformation, which contains several looped out bases. In contrast, the base-pairing scheme found for the isolated loop contains three mismatched base-pairs: an A+-C, a G-U wobble, and a sheared G-A base-pair and no looped out bases. Dynamics observed within the active site loop provide insight into the mechanism by which the RNA can rearrange its secondary structure into an "activated" conformation prior to cleavage. These findings lend support to the idea that RNA secondary structure is more fluid than once believed and that a better understanding of structure and dynamic features of ribozymes is required to unravel the intricacies of their catalytic abilities.

PubMed: 15328609
DOI: 10.1016/j.jmb.2004.06.084

実験手法

SOLUTION NMR