1TJD

The crystal structure of the reduced disulphide bond isomerase, DsbC, from Escherichia coli

Summary for 1TJD

• Entry DOI: 10.2210/pdb1tjd/pdb
• Descriptor: Thiol:disulfide interchange protein dsbC (2 entities in total)
• Functional Keywords: dimer, isomerase
• Biological source: Escherichia coli
• Cellular location: Periplasm: P21892
• Total number of polymer chains: 1
• Total formula weight: 23488.02

Authors

Banaszak, K.,Mechin, I.,Frost, G.,Rypniewski, W. (deposition date: 2004-06-04, release date: 2004-10-05, Last modification date: 2024-10-30)

Primary citation

Banaszak, K.,Mechin, I.,Frost, G.,Rypniewski, W.
Structure of the reduced disulfide-bond isomerase DsbC from Escherichia coli.
Acta Crystallogr.,Sect.D, 60:1747-1752, 2004

PubMed Abstract: Disufide-bond isomerase (DsbC) plays a crucial role in folding periplasmically excreted bacterial proteins. The crystal structure of the reduced form of DsbC is presented. The pair of thiol groups from Cys98 and Cys101 that form the reversible disulfide bond in the enzymatic active site are 3.1 A apart and the electron density clearly shows that the S atoms do not form a covalent bond. The other pair of Cys residues (141 and 163) in DsbC form a disulfide bond. This is different from the previously reported crystal form of DsbC (McCarthy et al., 2000), in which both Cys pairs are oxidized. Specific hydrogen-bond interactions are identified that stabilize the active site in the reactive reduced state with the special participation of hydrogen bonds between the active-site cysteine residues (98 and 101) and threonine residues 94 and 182. The present structure also differs in the orientation of the catalytic domains within the protein dimer. This is evidence of flexibility within the protein that probably plays a role in accommodating the substrates in the cleft between the catalytic domains.

PubMed: 15388920
DOI: 10.1107/S0907444904018359

Experimental method

X-RAY DIFFRACTION (2.5 Å)