1TG7

Native structure of beta-galactosidase from Penicillium sp.

1TG7 の概要

• エントリーDOI: 10.2210/pdb1tg7/pdb
• 関連するPDBエントリー: 1BGL 1KWG
• 分子名称: beta-galactosidase, 1,2-ETHANEDIOL, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (11 entities in total)
• 機能のキーワード: tim barrel domain, glycoside hydrolase, family gh35, glycoprotein, penicillium, hydrolase
• 由来する生物種: Penicillium sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 111849.98

構造登録者

Rojas, A.L.,Nagem, R.A.P.,Neustroev, K.N.,Arand, M.,Adamska, M.,Eneyskaya, E.V.,Kulminskaya, A.A.,Garratt, R.C.,Golubev, A.M.,Polikarpov, I. (登録日: 2004-05-28, 公開日: 2004-11-02, 最終更新日: 2024-10-30)

主引用文献

Rojas, A.L.,Nagem, R.A.P.,Neustroev, K.N.,Arand, M.,Adamska, M.,Eneyskaya, E.V.,Kulminskaya, A.A.,Garratt, R.C.,Golubev, A.M.,Polikarpov, I.
Crystal Structures of beta-Galactosidase from Penicillium sp. and its Complex with Galactose
J.Mol.Biol., 343:1281-1292, 2004

PubMed Abstract: Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. Here we report the crystallographic structures of Penicillium sp. beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectively. The amino acid sequence of this 120 kDa protein was first assigned putatively on the basis of inspection of the experimental electron density maps and then determined by nucleotide sequence analysis. Primary structure alignments reveal that Penicillium sp. beta-galactosidase belongs to family 35 of glycosyl hydrolases (GHF-35). This model is the first 3D structure for a member of GHF-35. Five distinct domains which comprise the structure are assembled in a way previously unobserved for beta-galactosidases. Superposition of this complex with other beta-galactosidase complexes from several hydrolase families allowed the identification of residue Glu200 as the proton donor and residue Glu299 as the nucleophile involved in catalysis. Penicillium sp. beta-galactosidase is a glycoprotein containing seven N-linked oligosaccharide chains and is the only structure of a glycosylated beta-galactosidase described to date.

PubMed: 15491613
DOI: 10.1016/j.jmb.2004.09.012

実験手法

X-RAY DIFFRACTION (1.9 Å)