1TFR

RNASE H FROM BACTERIOPHAGE T4

1TFR の概要

• エントリーDOI: 10.2210/pdb1tfr/pdb
• 分子名称: T4 RNASE H, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: 5u-3u exonuclease, rna:rna, dna:dna, metal-dependent, magnesium-containing, hydrolase (nucleic acid), hydrolase
• 由来する生物種: Enterobacteria phage T4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35659.52

構造登録者

Mueser, T.C.,Nossal, N.G.,Hyde, C.C. (登録日: 1996-04-27, 公開日: 1996-11-08, 最終更新日: 2024-02-14)

主引用文献

Mueser, T.C.,Nossal, N.G.,Hyde, C.C.
Structure of bacteriophage T4 RNase H, a 5' to 3' RNA-DNA and DNA-DNA exonuclease with sequence similarity to the RAD2 family of eukaryotic proteins.
Cell(Cambridge,Mass.), 85:1101-1112, 1996

PubMed Abstract: Bacteriophage T4 RNase H is a 5' to 3' exonuclease that removes RNA primers from the lagging strand of the DNA replication fork and is a member of the RAD2 family of eukaryotic and prokaryotic replication and repair nucleases. The crystal structure of the full-length native form of T4 RNase H has been solved at 2.06 angstroms resolution in the presence of Mg2+ but in the absence of nucleic acids. The most conserved residues are clustered together in a large cleft with two Mg2+ in the proposed active site. This structure suggests the way in which the widely separated conserved regions in the larger nucleotide excision repair proteins, such as human XPG, could assemble into a structure like that of the smaller replication nucleases.

PubMed: 8674116
DOI: 10.1016/S0092-8674(00)81310-0

実験手法

X-RAY DIFFRACTION (2.06 Å)