1TF5

Crystal structure of SecA in an open conformation from Bacillus Subtilis

1TF5 の概要

• エントリーDOI: 10.2210/pdb1tf5/pdb
• 関連するPDBエントリー: 1TF2
• 分子名称: Preprotein translocase secA subunit (2 entities in total)
• 機能のキーワード: atpase, helicase, translocation, secretion, protein transport
• 由来する生物種: Bacillus subtilis
• 細胞内の位置: Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): P28366
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 95956.09

構造登録者

Osborne, A.R.,Clemons Jr., W.M.,Rapoport, T.A. (登録日: 2004-05-26, 公開日: 2004-08-03, 最終更新日: 2023-08-23)

主引用文献

Osborne, A.R.,Clemons Jr., W.M.,Rapoport, T.A.
A large conformational change of the translocation ATPase SecA.
Proc.Natl.Acad.Sci.USA, 101:10937-10942, 2004

PubMed Abstract: The ATPase SecA mediates the posttranslational translocation of a wide range of polypeptide substrates through the SecY channel in the cytoplasmic membrane of bacteria. We have determined the crystal structure of a monomeric form of Bacillus subtilis SecA at a 2.2-A resolution. A comparison with the previously determined structures of SecA reveals a nucleotide-independent, large conformational change that opens a deep groove similar to that in other proteins that interact with diverse polypeptides. We propose that the open form of SecA represents an activated state.

PubMed: 15256599
DOI: 10.1073/pnas.0401742101

実験手法

X-RAY DIFFRACTION (2.18 Å)