1TEX

Mycobacterium smegmatis Stf0 Sulfotransferase with Trehalose

1TEX の概要

• エントリーDOI: 10.2210/pdb1tex/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900006
• 分子名称: Stf0 Sulfotransferase, alpha-D-glucopyranose-(1-1)-alpha-D-glucopyranose (3 entities in total)
• 機能のキーワード: mycobacterium, sulfotransferase, sulfolipid, sulfation, trehalose, trehalose-2-sulfate, transferase
• 由来する生物種: Mycobacterium smegmatis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 131359.22

構造登録者

Mougous, J.D.,Petzold, C.J.,Senaratne, R.H.,Lee, D.H.,Akey, D.L.,Lin, F.L.,Munchel, S.E.,Pratt, M.R.,Riley, L.W.,Leary, J.A.,Berger, J.M.,Bertozzi, C.R. (登録日: 2004-05-25, 公開日: 2004-07-20, 最終更新日: 2024-02-14)

主引用文献

Mougous, J.D.,Petzold, C.J.,Senaratne, R.H.,Lee, D.H.,Akey, D.L.,Lin, F.L.,Munchel, S.E.,Pratt, M.R.,Riley, L.W.,Leary, J.A.,Berger, J.M.,Bertozzi, C.R.
Identification, function and structure of the mycobacterial sulfotransferase that initiates sulfolipid-1 biosynthesis.
Nat.Struct.Mol.Biol., 11:721-729, 2004

PubMed Abstract: Sulfolipid-1 (SL-1) is an abundant sulfated glycolipid and potential virulence factor found in Mycobacterium tuberculosis. SL-1 consists of a trehalose-2-sulfate (T2S) disaccharide elaborated with four lipids. We identified and characterized a conserved mycobacterial sulfotransferase, Stf0, which generates the T2S moiety of SL-1. Biochemical studies demonstrated that the enzyme requires unmodified trehalose as substrate and is sensitive to small structural perturbations of the disaccharide. Disruption of stf0 in Mycobacterium smegmatis and M. tuberculosis resulted in the loss of T2S and SL-1 formation, respectively. The structure of Stf0 at a resolution of 2.6 A reveals the molecular basis of trehalose recognition and a unique dimer configuration that encloses the substrate into a bipartite active site. These data provide strong evidence that Stf0 carries out the first committed step in the biosynthesis of SL-1 and establish a system for probing the role of SL-1 in M. tuberculosis infection.

PubMed: 15258569
DOI: 10.1038/nsmb802

実験手法

X-RAY DIFFRACTION (2.6 Å)