1TER

SOLUTION STRUCTURE OF TERTIAPIN DETERMINED USING NUCLEAR MAGNETIC RESONANCE AND DISTANCE GEOMETRY

1TER の概要

• エントリーDOI: 10.2210/pdb1ter/pdb
• 分子名称: TERTIAPIN (1 entity in total)
• 機能のキーワード: toxin
• 由来する生物種: Apis mellifera (honey bee)
• 細胞内の位置: Secreted: P56587
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2464.14

構造登録者

Xu, X.,Nelson, J.W. (登録日: 1994-04-08, 公開日: 1995-02-07, 最終更新日: 2024-11-20)

主引用文献

Xu, X.,Nelson, J.W.
Solution structure of tertiapin determined using nuclear magnetic resonance and distance geometry.
Proteins, 17:124-137, 1993

PubMed Abstract: The solution structure of tertiapin, a 21-residue bee venom peptide, has been characterized by circular dichroism (CD), two-dimensional nuclear magnetic resonance (NMR) spectroscopy, and distance geometry. A total of 21 lowest error structures were obtained from distance geometry calculations. Superimposition of these structures shows that the backbone of tertiapin is very well defined. One type-I reverse turn from residue 4 to 7 and an alpha-helix from residue 12 to 19 exist in the structure of tertiapin. The alpha-helical region is best defined from both conformational analysis and structural superimposition. The overall three-dimensional structure of tertiapin is highly compact resulting from side chain interactions. The structural information obtained from CD and NMR are compared for both tertiapin and apamin (ref. 3), another bee venom peptide. Tertiapin and apamin have some similar secondary structure, but display different tertiary structures.

PubMed: 8265561
DOI: 10.1002/prot.340170203

実験手法

SOLUTION NMR