1TE4

Solution structure of MTH187. Ontario Centre for Structural Proteomics target MTH0187_1_111; Northeast Structural Genomics Target TT740

1TE4 の概要

• エントリーDOI: 10.2210/pdb1te4/pdb
• 分子名称: conserved protein MTH187 (1 entity in total)
• 機能のキーワード: mth187, methanobacterium thermoautotrophicum, structural proteomics, heat-like repeat, structural genomics, ocsp, nesg, protein structure initiative, psi, northeast structural genomics consortium, unknown function
• 由来する生物種: Methanothermobacter thermautotrophicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14582.25

構造登録者

Gignac, I.,Julien, O.,Yee, A.,Arrowsmith, C.H.,Gagne, S.M.,Northeast Structural Genomics Consortium (NESG) (登録日: 2004-05-24, 公開日: 2004-07-13, 最終更新日: 2024-05-22)

主引用文献

Julien, O.,Gignac, I.,Hutton, A.,Yee, A.,Arrowsmith, C.H.,Gagne, S.M.
MTH187 from Methanobacterium thermoautotrophicum has three HEAT-like Repeats.
J.Biomol.Nmr, 35:149-154, 2006

PubMed Abstract: With the completion of genome sequencing projects, there are a large number of proteins for which we have little or no functional information. Since protein function is closely related to three-dimensional conformation, structural proteomics is one avenue where the role of proteins with unknown function can be investigated. In the present structural project, the structure of MTH187 has been determined by solution-state NMR spectroscopy. This protein of 12.4 kDa is one of the 424 non-membrane proteins that were cloned and purified for the structural proteomic project of Methanobacterium thermoautotrophicum [Christendat, D., Yee, A., Dharamsi, A., Kluger, Y., Gerstein, M., Arrowsmith, C.H. and Edwards, A.M. (2000) Prog. Biophys. Mol. Biol., 73, 339-345]. Methanobacterium thermoautotrophicum is a thermophilic archaeon that grows optimally at 65 degrees C. A particular characteristic of this microorganism is its ability to generate methane from carbon dioxide and hydrogen [Smith, D.R., Doucette-Stamm, L.A., Deloughery, C., Lee, H., Dubois, J., Aldredge, T., Bashirzadeh, R., Blakely, D., Cook, R., Gilbert, K., Harrison, D., Hoang, L., Keagle, P., Lumm, W., Pothier, B., Qiu, D., Spadafora, R., Vicaire, R., Wang, Y., Wierzbowski, J., Gibson, R., Jiwani, N., Caruso, A., Bush, D., Reeve, J. N. et al. (1997) J. Bacteriol., 179, 7135-7155].

PubMed: 16819590
DOI: 10.1007/s10858-006-0029-3

実験手法

SOLUTION NMR