1TCR

MURINE T-CELL ANTIGEN RECEPTOR 2C CLONE

1TCR の概要

• エントリーDOI: 10.2210/pdb1tcr/pdb
• 分子名称: ALPHA, BETA T-CELL RECEPTOR (VB8.2DB2JB2.4CB2\;VA3JA58CA), beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: t-cell, receptor, transmembrane, glycoprotein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50820.09

構造登録者

Garcia, K.C.,Degano, M.,Stanfield, R.L.,Wilson, I.A. (登録日: 1996-09-12, 公開日: 1997-03-12, 最終更新日: 2024-10-23)

主引用文献

Garcia, K.C.,Degano, M.,Stanfield, R.L.,Brunmark, A.,Jackson, M.R.,Peterson, P.A.,Teyton, L.,Wilson, I.A.
An alphabeta T cell receptor structure at 2.5 A and its orientation in the TCR-MHC complex.
Science, 274:209-219, 1996

PubMed Abstract: The central event in the cellular immune response to invading microorganisms is the specific recognition of foreign peptides bound to major histocompatibility complex (MHC) molecules by the alphabeta T cell receptor (TCR). The x-ray structure of the complete extracellular fragment of a glycosylated alphabeta TCR was determined at 2.5 angstroms, and its orientation bound to a class I MHC-peptide (pMHC) complex was elucidated from crystals of the TCR-pMHC complex. The TCR resembles an antibody in the variable Valpha and Vbeta domains but deviates in the constant Calpha domain and in the interdomain pairing of Calpha with Cbeta. Four of seven possible asparagine-linked glycosylation sites have ordered carbohydrate moieties, one of which lies in the Calpha-Cbeta interface. The TCR combining site is relatively flat except for a deep hydrophobic cavity between the hypervariable CDR3s (complementarity-determining regions) of the alpha and beta chains. The 2C TCR covers the class I MHC H-2Kb binding groove so that the Valpha CDRs 1 and 2 are positioned over the amino-terminal region of the bound dEV8 peptide, the Vbeta chain CDRs 1 and 2 are over the carboxyl-terminal region of the peptide, and the Valpha and Vbeta CDR3s straddle the peptide between the helices around the central position of the peptide.

PubMed: 8824178
DOI: 10.1126/science.274.5285.209

実験手法

X-RAY DIFFRACTION (2.5 Å)