1TBK

NMR structure of the VS ribozyme stem-loop V RNA in the absence of multivalent ions.

1TBK の概要

• エントリーDOI: 10.2210/pdb1tbk/pdb
• 分子名称: VS ribozyme stem-loop V (1 entity in total)
• 機能のキーワード: u-turn; hairpin, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5419.26

構造登録者

Campbell, D.O.,Legault, P. (登録日: 2004-05-20, 公開日: 2005-03-01, 最終更新日: 2024-05-22)

主引用文献

Campbell, D.O.,Legault, P.
Nuclear Magnetic Resonance Structure of the Varkud Satellite Ribozyme Stem-Loop V RNA and Magnesium-Ion Binding from Chemical-Shift Mapping
Biochemistry, 44:4157-4170, 2005

PubMed Abstract: An important step in the substrate recognition of the Neurospora Varkud Satellite (VS) ribozyme is the formation of a magnesium-dependent loop/loop interaction between the terminal loops of stem-loops I and V. We have studied the structure of stem-loop V by nuclear magnetic resonance spectroscopy and shown that it adopts a U-turn conformation, a common motif found in RNA. Structural comparisons indicate that the U-turn of stem-loop V fulfills some but not all of the structural characteristics found in canonical U-turn structures. This U-turn conformation exposes the Watson-Crick faces of the bases within stem-loop V (G697, A698, and C699) and makes them accessible for interaction with stem-loop I. Using chemical-shift mapping, we show that magnesium ions interact with the loop of the isolated stem-loop V and induce a conformational change that may be important for interaction with stem-loop I. This study expands our understanding of the role of U-turn motifs in RNA structure and function and provides insights into the mechanism of substrate recognition in the VS ribozyme.

PubMed: 15766243
DOI: 10.1021/bi047963l

実験手法

SOLUTION NMR