1TBA

SOLUTION STRUCTURE OF A TBP-TAFII230 COMPLEX: PROTEIN MIMICRY OF THE MINOR GROOVE SURFACE OF THE TATA BOX UNWOUND BY TBP, NMR, 25 STRUCTURES

1TBA の概要

• エントリーDOI: 10.2210/pdb1tba/pdb
• NMR情報: BMRB: 4223
• 分子名称: TRANSCRIPTION INITIATION FACTOR IID 230K CHAIN, TRANSCRIPTION INITIATION FACTOR TFIID (2 entities in total)
• 機能のキーワード: complex (transcription factors), tbp, taf, tafii230, tfiid, solution structure
• 由来する生物種: Drosophila melanogaster (fruit fly); 詳細
• 細胞内の位置: Nucleus: P51123 P13393
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27133.43

構造登録者

Liu, D.,Ishima, R.,Tong, K.I.,Bagby, S.,Kokubo, T.,Muhandiram, D.R.,Kay, L.E.,Nakatani, Y.,Ikura, M. (登録日: 1998-08-16, 公開日: 1999-08-16, 最終更新日: 2024-05-22)

主引用文献

Liu, D.,Ishima, R.,Tong, K.I.,Bagby, S.,Kokubo, T.,Muhandiram, D.R.,Kay, L.E.,Nakatani, Y.,Ikura, M.
Solution structure of a TBP-TAF(II)230 complex: protein mimicry of the minor groove surface of the TATA box unwound by TBP.
Cell(Cambridge,Mass.), 94:573-583, 1998

PubMed Abstract: General transcription factor TFIID consists of TATA box-binding protein (TBP) and TBP-associated factors (TAF(II)s), which together play a central role in both positive and negative regulation of transcription. The N-terminal region of the 230 kDa Drosophila TAF(II) (dTAF(II)230) binds directly to TBP and inhibits TBP binding to the TATA box. We report here the solution structure of the complex formed by dTAF(II)230 N-terminal region (residues 11-77) and TBP. dTAF(II)230(11-77) comprises three alpha helices and a beta hairpin, forming a core that occupies the concave DNA-binding surface of TBP. The TBP-binding surface of dTAF(II)230 markedly resembles the minor groove surface of the partially unwound TATA box in the TBP-TATA complex. This protein mimicry of the TATA element surface provides the structural basis of the mechanism by which dTAF(II)230 negatively controls the TATA box-binding activity within the TFIID complex.

PubMed: 9741622
DOI: 10.1016/S0092-8674(00)81599-8

実験手法

SOLUTION NMR