1T9J

I-CreI(Q47E)/DNA complex

1T9J の概要

• エントリーDOI: 10.2210/pdb1t9j/pdb
• 関連するPDBエントリー: 1G9Y 1G9Z 1T9I
• 分子名称: 5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*TP*CP*G)-3', 5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*TP*GP*C)-3', DNA endonuclease I-CreI, ... (4 entities in total)
• 機能のキーワード: protein, dna, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Chlamydomonas reinhardtii
• 細胞内の位置: Plastid, chloroplast: P05725
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 52186.62

構造登録者

Chevalier, B.,Sussman, D.,Otis, C.,Boudreau, D.,Turmel, M.,Lemieux, C.,Stephens, K.,Monnat Jr., R.J.,Stoddard, B.L. (登録日: 2004-05-17, 公開日: 2004-11-16, 最終更新日: 2023-08-23)

主引用文献

Chevalier, B.,Sussman, D.,Otis, C.,Noel, A.J.,Turmel, M.,Lemieux, C.,Stephens, K.,Monnat Jr., R.J.,Stoddard, B.L.
Metal-Dependent DNA Cleavage Mechanism of the I-CreI LAGLIDADG Homing Endonuclease.
Biochemistry, 43:14015-14026, 2004

PubMed Abstract: The LAGLIDADG homing endonucleases include free-standing homodimers, pseudosymmetric monomers, and related enzyme domains embedded within inteins. DNA-bound structures of homodimeric I-CreI and monomeric I-SceI indicate that three catalytic divalent metal ions are distributed across a pair of overlapping active sites, with one shared metal participating in both strand cleavage reactions. These structures differ in the precise position and binding interactions of the metals. We have studied the metal dependence for the I-CreI homodimer using site-directed mutagenesis of active site residues and assays of binding affinity and cleavage activity. We have also reassessed the binding of a nonactivating metal ion (calcium) in the wild-type enzyme-substrate complex, and determined the DNA-bound structure of two inactive enzyme mutants. The conclusion of these studies is that the catalytic mechanism of symmetric LAGLIDADG homing endonucleases, and probably many of their monomeric cousins, involves a canonical two-metal mechanism in each of two active sites, which are chemically and structurally tethered to one another by a shared metal ion. Failure to occupy the shared metal site, as observed in the presence of calcium or when the metal-binding side chain from the LAGLIDADG motif (Asp 20) is mutated to asparagine, prevents cleavage by the enzyme.

PubMed: 15518550
DOI: 10.1021/bi048970c

実験手法

X-RAY DIFFRACTION (2 Å)