1T4W

Structural Differences in the DNA Binding Domains of Human p53 and its C. elegans Ortholog Cep-1: Structure of C. elegans Cep-1

1T4W の概要

• エントリーDOI: 10.2210/pdb1t4w/pdb
• 分子名称: C.Elegans p53 tumor suppressor-like transcription factor, ZINC ION (3 entities in total)
• 機能のキーワード: dna-binding domain, transcription
• 由来する生物種: Caenorhabditis elegans
• 細胞内の位置: Nucleus: Q20646
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23608.45

構造登録者

Huyen, Y.,Jeffrey, P.D.,Derry, W.B.,Rothman, J.H.,Pavletich, N.P.,Stavridi, E.S.,Halazonetis, T.D. (登録日: 2004-04-30, 公開日: 2004-07-20, 最終更新日: 2024-02-14)

主引用文献

Huyen, Y.,Jeffrey, P.D.,Derry, W.B.,Rothman, J.H.,Pavletich, N.P.,Stavridi, E.S.,Halazonetis, T.D.
Structural Differences in the DNA Binding Domains of Human p53 and Its C. elegans Ortholog Cep-1.
Structure, 12:1237-1243, 2004

PubMed Abstract: The DNA binding domains of human p53 and Cep-1, its C. elegans ortholog, recognize essentially identical DNA sequences despite poor sequence similarity. We solved the three-dimensional structure of the Cep-1 DNA binding domain in the absence of DNA and compared it to that of human p53. The two domains have similar overall folds. However, three loops, involved in DNA and Zn binding in human p53, contain small alpha helices in Cep-1. The alpha helix in loop L3 of Cep-1 orients the side chains of two conserved arginines toward DNA; in human p53, both arginines are mutation hotspots, but only one contacts DNA. The alpha helix in loop L1 of Cep-1 repositions the entire loop, making it unlikely for residues of this loop to contact bases in the major groove of DNA, as occurs in human p53. Thus, during evolution there have been considerable changes in the structure of the p53 DNA binding domain.

PubMed: 15242600
DOI: 10.1016/j.str.2004.05.007

実験手法

X-RAY DIFFRACTION (2.1 Å)