1T1O

Components of the control 70S ribosome to provide reference for the RRF binding site

1T1O の概要

• エントリーDOI: 10.2210/pdb1t1o/pdb
• 関連するPDBエントリー: 1FJF 1KC9 1T1M
• EMDBエントリー: 1077
• 分子名称: dodecamer fragment of double helix from 23S rRNA, 19-mer fragment of the 23S rRNA, 42-mer fragment of double helix from 16S rRNA (3 entities in total)
• 機能のキーワード: rrf binding position on the ribosome, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 23511.14

構造登録者

Agrawal, R.K.,Sharma, M.R.,Kiel, M.C.,Hirokawa, G.,Booth, T.M.,Spahn, C.M.,Grassucci, R.A.,Kaji, A.,Frank, J. (登録日: 2004-04-16, 公開日: 2004-06-15, 最終更新日: 2024-02-14)

主引用文献

Agrawal, R.K.,Sharma, M.R.,Kiel, M.C.,Hirokawa, G.,Booth, T.M.,Spahn, C.M.,Grassucci, R.A.,Kaji, A.,Frank, J.
Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: Functional implications
Proc.Natl.Acad.Sci.USA, 101:8900-8905, 2004

PubMed Abstract: After the termination step of protein synthesis, a deacylated tRNA and mRNA remain associated with the ribosome. The ribosome-recycling factor (RRF), together with elongation factor G (EF-G), disassembles this posttermination complex into mRNA, tRNA, and the ribosome. We have obtained a three-dimensional cryo-electron microscopic map of a complex of the Escherichia coli 70S ribosome and RRF. We find that RRF interacts mainly with the segments of the large ribosomal subunit's (50S) rRNA helices that are involved in the formation of two central intersubunit bridges, B2a and B3. The binding of RRF induces considerable conformational changes in some of the functional domains of the ribosome. As compared to its binding position derived previously by hydroxyl radical probing study, we find that RRF binds further inside the intersubunit space of the ribosome such that the tip of its domain I is shifted (by approximately 13 A) toward protein L5 within the central protuberance of the 50S subunit, and domain II is oriented more toward the small ribosomal subunit (30S). Overlapping binding sites of RRF, EF-G, and the P-site tRNA suggest that the binding of EF-G would trigger the removal of deacylated tRNA from the P site by moving RRF toward the ribosomal E site, and subsequent removal of mRNA may be induced by a shift in the position of 16S rRNA helix 44, which harbors part of the mRNA.

PubMed: 15178758
DOI: 10.1073/pnas.0401904101

実験手法

ELECTRON MICROSCOPY (12 Å)