1SY9

Structure of calmodulin complexed with a fragment of the olfactory CNG channel

1SY9 の概要

• エントリーDOI: 10.2210/pdb1sy9/pdb
• NMR情報: BMRB: 5480
• 分子名称: CALMODULIN, Cyclic-nucleotide-gated olfactory channel, CALCIUM ION (3 entities in total)
• 機能のキーワード: 4 helix-turn-helix, calcium-binding protein
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• 細胞内の位置: Membrane; Multi-pass membrane protein: Q03041
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20151.48

構造登録者

Contessa, G.M.,Orsale, M.,Melino, S.,Torre, V.,Paci, M.,Desideri, A.,Cicero, D.O. (登録日: 2004-04-01, 公開日: 2005-04-12, 最終更新日: 2024-05-22)

主引用文献

Contessa, G.M.,Orsale, M.,Melino, S.,Torre, V.,Paci, M.,Desideri, A.,Cicero, D.O.
Structure of calmodulin complexed with an olfactory CNG channel fragment and role of the central linker: residual dipolar couplings to evaluate calmodulin binding modes outside the kinase family.
J.Biomol.Nmr, 31:185-199, 2005

PubMed Abstract: The NMR high-resolution structure of calmodulin complexed with a fragment of the olfactory cyclic-nucleotide gated channel is described. This structure shows features that are unique for this complex, including an active role of the linker connecting the N- and C-lobes of calmodulin upon binding of the peptide. Such linker is not only involved in the formation of an hydrophobic pocket to accommodate a bulky peptide residue, but it also provides a positively charged region complementary to a negative charge of the target. This complex of calmodulin with a target not belonging to the kinase family was used to test the residual dipolar coupling (RDC) approach for the determination of calmodulin binding modes to peptides. Although the complex here characterized belongs to the (1--14) family, high Q values were obtained with all the 1:1 complexes for which crystalline structures are available. Reduction of the RDC data set used for the correlation analysis to structured regions of the complex allowed a clear identification of the binding mode. Excluded regions comprise calcium binding loops and loops connecting the EF-hand motifs.

PubMed: 15803393
DOI: 10.1007/s10858-005-0165-1

実験手法

SOLUTION NMR