1SXE
The solution structure of the Pointed (PNT) domain from the transcrition factor Erg
Summary for 1SXE
| Entry DOI | 10.2210/pdb1sxe/pdb |
| NMR Information | BMRB: 5399 |
| Descriptor | Transcriptional regulator ERG (1 entity in total) |
| Functional Keywords | alpha helical, transcription, signaling protein |
| Biological source | Homo sapiens (human) |
| Cellular location | Nucleus: P11308 |
| Total number of polymer chains | 1 |
| Total formula weight | 11395.99 |
| Authors | Mackereth, C.D.,Schaerpf, M.,Gentile, L.N.,MacIntosh, S.E.,Slupsky, C.M.,McIntosh, L.P. (deposition date: 2004-03-30, release date: 2004-09-21, Last modification date: 2024-05-22) |
| Primary citation | Mackereth, C.D.,Schaerpf, M.,Gentile, L.N.,MacIntosh, S.E.,Slupsky, C.M.,McIntosh, L.P. Diversity in Structure and Function of the Ets Family PNT Domains. J.Mol.Biol., 342:1249-1264, 2004 Cited by PubMed Abstract: The PNT (or Pointed) domain, present within a subset of the Ets family of transcription factors, is structurally related to the larger group of SAM domains through a common tertiary arrangement of four alpha-helices. Previous studies have shown that, in contrast to the PNT domain from Tel, this domain from Ets-1 contains an additional N-terminal helix integral to its folded structure. To further investigate the structural plasticity of the PNT domain, we have used NMR spectroscopy to characterize this domain from two additional Ets proteins, Erg and GABPalpha. These studies both define the conserved and variable features of the PNT domain, and demonstrate that the additional N-terminal helix is also present in GABPalpha, but not Erg. In contrast to Tel and Yan, which self-associate to form insoluble polymers, we also show that the isolated PNT domains from Ets-1, Ets-2, Erg, Fli-1, GABPalpha, and Pnt-P2 are monomeric in solution. Furthermore, these soluble PNT domains do not associate in any pair-wise combination. Thus these latter Ets family PNT domains likely mediate interactions with additional components of the cellular signaling or transcriptional machinery. PubMed: 15351649DOI: 10.1016/j.jmb.2004.07.094 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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