1SW7

Triosephosphate isomerase from Gallus gallus, loop 6 mutant K174N, T175S, A176S

1SW7 の概要

• エントリーDOI: 10.2210/pdb1sw7/pdb
• 関連するPDBエントリー: 1SPQ 1SQ7 1SSD 1SSG 1SU5 1SW0 1SW3
• 分子名称: Triosephosphate isomerase, 2-PHOSPHOGLYCOLIC ACID (3 entities in total)
• 機能のキーワード: tim barrel, flexible loop, hinge, isomerase
• 由来する生物種: Gallus gallus (chicken)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53600.77

構造登録者

Kursula, I.,Salin, M.,Sun, J.,Norledge, B.V.,Haapalainen, A.M.,Sampson, N.S.,Wierenga, R.K. (登録日: 2004-03-30, 公開日: 2004-08-24, 最終更新日: 2023-10-25)

主引用文献

Kursula, I.,Salin, M.,Sun, J.,Norledge, B.V.,Haapalainen, A.M.,Sampson, N.S.,Wierenga, R.K.
Understanding protein lids: structural analysis of active hinge mutants in triosephosphate isomerase
Protein Eng.Des.Sel., 17:375-382, 2004

PubMed Abstract: The conformational switch from open to closed of the flexible loop 6 of triosephosphate isomerase (TIM) is essential for the catalytic properties of TIM. Using a directed evolution approach, active variants of chicken TIM with a mutated C-terminal hinge tripeptide of loop 6 have been generated (Sun,J. and Sampson,N.S., Biochemistry, 1999, 38, 11474-11481). In chicken TIM, the wild-type C-terminal hinge tripeptide is KTA. Detailed enzymological characterization of six variants showed that some of these (LWA, NPN, YSL, KTK) have decreased catalytic efficiency, whereas others (KVA, NSS) are essentially identical with wild-type. The structural characterization of these six variants is reported. No significant structural differences compared with the wild-type are found for KVA, NSS and LWA, but substantial structural adaptations are seen for NPN, YSL and KTK. These structural differences can be understood from the buried position of the alanine side chain in the C-hinge position 3 in the open conformation of wild-type loop 6. Replacement of this alanine with a bulky side chain causes the closed conformation to be favored, which correlates with the decreased catalytic efficiency of these variants. The structural context of loop 6 and loop 7 and their sequence conservation in 133 wild-type sequences is also discussed.

PubMed: 15166315
DOI: 10.1093/protein/gzh048

実験手法

X-RAY DIFFRACTION (2.22 Å)