1SVR

STRUCTURE OF SEVERIN DOMAIN 2 IN SOLUTION

1SVR の概要

• エントリーDOI: 10.2210/pdb1svr/pdb
• 分子名称: SEVERIN (1 entity in total)
• 機能のキーワード: actin-binding
• 由来する生物種: Dictyostelium discoideum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12260.84

構造登録者

Schnuchel, A.,Holak, T.A. (登録日: 1994-10-12, 公開日: 1995-02-07, 最終更新日: 2017-11-29)

主引用文献

Schnuchel, A.,Wiltscheck, R.,Eichinger, L.,Schleicher, M.,Holak, T.A.
Structure of severin domain 2 in solution.
J.Mol.Biol., 247:21-27, 1995

PubMed Abstract: The three-dimensional structure of domain 2 of severin in aqueous solution was determined by nuclear magnetic resonance spectroscopy. Severin is a Ca(2+)-activated actin-binding protein that servers F-actin, nucleates actin assembly, and caps the fast-growing ends of actin filaments. The 114-residue domain consists of a central five-stranded beta-sheet, sandwiched between a parallel four-turn alpha-helix and, on the other face, a roughly perpendicular two-turn alpha-helix. There are two distinct binding sites for Ca2+ located near the N and C termini of the long helix. Conserved residues of the gelsolin-severin family contribute to the apolar core of domain 2 of severin, so that the overall fold of the protein is similar to those of segment 1 of gelsolin and profilins. Together with biochemical experiments, this structure helps to explain how severin interacts with actin.

PubMed: 7897658
DOI: 10.1006/jmbi.1994.0118

実験手法

SOLUTION NMR