1SVF

PARAMYXOVIRUS SV5 FUSION PROTEIN CORE

Summary for 1SVF

• Entry DOI: 10.2210/pdb1svf/pdb
• Descriptor: PROTEIN (FUSION GLYCOPROTEIN), CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: paramyxovirus, fusion, sv5, coiled-coil, viral protein
• Biological source: Simian virus 5 (strain W3); More
• Cellular location: Virion membrane; Single-pass type I membrane protein (By similarity): P04849 P04849
• Total number of polymer chains: 4
• Total formula weight: 21145.28

Authors

Baker, K.A.,Dutch, R.E.,Lamb, R.A.,Jardetzky, T.S. (deposition date: 1999-02-27, release date: 1999-03-26, Last modification date: 2023-12-27)

Primary citation

Baker, K.A.,Dutch, R.E.,Lamb, R.A.,Jardetzky, T.S.
Structural basis for paramyxovirus-mediated membrane fusion.
Mol.Cell, 3:309-319, 1999

PubMed Abstract: Paramyxoviruses are responsible for significant human mortality and disease worldwide, but the molecular mechanisms underlying their entry into host cells remain poorly understood. We have solved the crystal structure of a fragment of the simian parainfluenza virus 5 fusion protein (SV5 F), revealing a 96 A long coiled coil surrounded by three antiparallel helices. This structure places the fusion and transmembrane anchor of SV5 F in close proximity with a large intervening domain at the opposite end of the coiled coil. Six amino acids, potentially part of the fusion peptide, form a segment of the central coiled coil, suggesting that this structure extends into the membrane. Deletion mutants of SV5 F indicate that putative flexible tethers between the coiled coil and the viral membrane are dispensable for fusion. The lack of flexible tethers may couple a final conformational change in the F protein directly to the fusion of two bilayers.

PubMed: 10198633
DOI: 10.1016/S1097-2765(00)80458-X

Experimental method

X-RAY DIFFRACTION (1.4 Å)