1SSO

SOLUTION STRUCTURE AND DNA-BINDING PROPERTIES OF A THERMOSTABLE PROTEIN FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS

1SSO の概要

• エントリーDOI: 10.2210/pdb1sso/pdb
• NMR情報: BMRB: 5909,5910
• 分子名称: SSO7D (1 entity in total)
• 機能のキーワード: dna-binding protein, dna binding protein
• 由来する生物種: Sulfolobus solfataricus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7034.22

構造登録者

Baumann, H.,Knapp, S.,Lundback, T.,Ladenstein, R.,Hard, T. (登録日: 1995-03-31, 公開日: 1995-05-08, 最終更新日: 2024-05-22)

主引用文献

Baumann, H.,Knapp, S.,Lundback, T.,Ladenstein, R.,Hard, T.
Solution structure and DNA-binding properties of a thermostable protein from the archaeon Sulfolobus solfataricus.
Nat.Struct.Biol., 1:808-819, 1994

PubMed Abstract: The archaeon Sulfolobus solfataricus expresses large amounts of a small basic protein, Sso7d, which was previously identified as a DNA-binding protein possibly involved in compaction of DNA. We have determined the solution structure of Sso7d. The protein consists of a triple-stranded anti-parallel beta-sheet onto which an orthogonal double-stranded beta-sheet is packed. This topology is very similar to that found in eukaryotic Src homology-3 (SH3) domains. Sso7d binds strongly (Kd < 10 microM) to double-stranded DNA and protects it from thermal denaturation. In addition, we note that epsilon-mono-methylation of lysine side chains of Sso7d is governed by cell growth temperatures, suggesting that methylation is related to the heat-shock response.

PubMed: 7634092
DOI: 10.1038/nsb1194-808

実験手法

SOLUTION NMR