1SSL

Solution structure of the PSI domain from the Met receptor

1SSL の概要

• エントリーDOI: 10.2210/pdb1ssl/pdb
• NMR情報: BMRB: 6165
• 分子名称: Hepatocyte growth factor receptor (1 entity in total)
• 機能のキーワード: cysteine knot, structural protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P08581
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5315.10

構造登録者

Kozlov, G.,Perreault, A.,Schrag, J.D.,Cygler, M.,Gehring, K.,Ekiel, I. (登録日: 2004-03-24, 公開日: 2004-10-12, 最終更新日: 2024-10-30)

主引用文献

Kozlov, G.,Perreault, A.,Schrag, J.D.,Park, M.,Cygler, M.,Gehring, K.,Ekiel, I.
Insights into function of PSI domains from structure of the Met receptor PSI domain.
Biochem.Biophys.Res.Commun., 321:234-240, 2004

PubMed Abstract: PSI domains are cysteine-rich modules found in extracellular fragments of hundreds of signaling proteins, including plexins, semaphorins, integrins, and attractins. Here, we report the solution structure of the PSI domain from the human Met receptor, a receptor tyrosine kinase critical for proliferation, motility, and differentiation. The structure represents a cysteine knot with short regions of secondary structure including a three-stranded antiparallel beta-sheet and two alpha-helices. All eight cysteines are involved in disulfide bonds with the pattern consistent with that for the PSI domain from Sema4D. Comparison with the Sema4D structure identifies a structurally conserved core comprising the N-terminal half of the PSI domain. Interestingly, this part links adjacent SEMA and immunoglobulin domains in the Sema4D structure, suggesting that the PSI domain serves as a wedge between propeller and immunoglobulin domains and is responsible for the correct positioning of the ligand-binding site of the receptor.

PubMed: 15358240
DOI: 10.1016/j.bbrc.2004.06.132

実験手法

SOLUTION NMR