1SSK

Structure of the N-terminal RNA-binding Domain of the SARS CoV Nucleocapsid Protein

1SSK の概要

• エントリーDOI: 10.2210/pdb1ssk/pdb
• 分子名称: Nucleocapsid protein (1 entity in total)
• 機能のキーワード: nucleocapsid protein, structural protein
• 由来する生物種: SARS coronavirus
• 細胞内の位置: Virion: P59595
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17179.03

構造登録者

Huang, Q.,Yu, L.,Petros, A.M.,Gunasekera, A.,Liu, Z.,Xu, N.,Hajduk, P.,Mack, J.,Fesik, S.W.,Olejniczak, E.T. (登録日: 2004-03-24, 公開日: 2004-06-08, 最終更新日: 2024-05-22)

主引用文献

Huang, Q.,Yu, L.,Petros, A.M.,Gunasekera, A.,Liu, Z.,Xu, N.,Hajduk, P.,Mack, J.,Fesik, S.W.,Olejniczak, E.T.
Structure of the N-Terminal RNA-Binding Domain of the SARS CoV Nucleocapsid Protein.
Biochemistry, 43:6059-6063, 2004

PubMed Abstract: The severe acute respiratory syndrome (SARS) virus belongs to the Coronaviridea family of viruses. Its virion encodes several proteins including a replicase and four structural proteins. Here we describe the three-dimensional structure of the N-terminal domain of the SARS coronavirus (CoV) nucleocapsid protein. The protein consists of a five-stranded beta sheet with a folding topology distinct from other RNA-binding proteins. Single-stranded RNAs bind to the protein surface at the junction between a flexible, positively charged beta hairpin and the core structure. NMR-based screening was used to identify low molecular weight compounds that bind to this site.

PubMed: 15147189
DOI: 10.1021/bi036155b

実験手法

SOLUTION NMR