1SOY

Solution structure of the bacterial frataxin orthologue, CyaY

1SOY の概要

• エントリーDOI: 10.2210/pdb1soy/pdb
• 分子名称: CyaY protein (1 entity in total)
• 機能のキーワード: frataxin, friedreich's ataxia iron binding, unknown function
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12370.49

構造登録者

Nair, M.,Adinolfi, S.,Pastore, C.,Kelly, G.,Temussi, P.,Pastore, A. (登録日: 2004-03-16, 公開日: 2004-11-23, 最終更新日: 2024-05-22)

主引用文献

Nair, M.,Adinolfi, S.,Pastore, C.,Kelly, G.,Temussi, P.,Pastore, A.
Solution Structure of the Bacterial Frataxin Ortholog, CyaY; Mapping the Iron Binding Sites
Structure, 12:2037-2048, 2004

PubMed Abstract: CyaY is the bacterial ortholog of frataxin, a small mitochondrial iron binding protein thought to be involved in iron sulphur cluster formation. Loss of frataxin function leads to the neurodegenerative disorder Friedreich's ataxia. We have solved the solution structure of CyaY and used the structural information to map iron binding onto the protein surface. Comparison of the behavior of wild-type CyaY with that of a mutant indicates that specific binding with a defined stoichiometry does not require aggregation and that the main binding site, which hosts both Fe(2+) and Fe(3+), occupies a highly anionic surface of the molecule. This function is conserved across species since the corresponding region of human frataxin is also able to bind iron, albeit with weaker affinity. The presence of secondary binding sites on CyaY, but not on frataxin, hints at a possible polymerization mechanism. We suggest mutations that may provide further insights into the frataxin function.

PubMed: 15530368
DOI: 10.1016/j.str.2004.08.012

実験手法

SOLUTION NMR