1SOH

The structure of human apolipoprotein C-II in dodecyl phosphocholine

1SOH の概要

• エントリーDOI: 10.2210/pdb1soh/pdb
• 関連するPDBエントリー: 1I5J
• 分子名称: Apolipoprotein C-II (1 entity in total)
• 機能のキーワード: lipid transport
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P02655
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8921.86

構造登録者

MacRaild, C.A.,Howlett, G.J.,Gooley, P.R. (登録日: 2004-03-14, 公開日: 2004-07-27, 最終更新日: 2024-05-22)

主引用文献

MacRaild, C.A.,Howlett, G.J.,Gooley, P.R.
The structure and interactions of human apolipoprotein C-II in dodecyl phosphocholine
Biochemistry, 43:8084-8093, 2004

PubMed Abstract: The structure of human apolipoprotein C-II (apoC-II) in the presence of dodecyl phosphocholine (DPC) micelles has been investigated by NMR spectroscopy. The resulting structural information is compared to that available for apoC-II in the presence of sodium dodecyl sulfate, revealing a high level of overall similarity but several significant differences. These findings further our understandings of the structural basis for apoC-II function. The interactions of the protein with the detergent micelle are probed using intermolecular nuclear Overhauser effects (NOEs) and paramagnetic agents. These interactions are seen across almost the full length of apoC-II and show the periodicity expected for an amphipathic helix interacting with the amphipathic surface of the DPC micelle. Furthermore, we observe specific contacts between lysine residues of apoC-II and protons near the phosphate group of DPC, consistent with the predictions of the so-called "snorkel hypothesis" of the structural basis for the apolipoprotein/lipid interaction (Segrest, J. P., Jackson, R. L., Morrisett, J. D., and Gotto, A. M., Jr. (1974) A molecular theory of lipid-protein interactions in the plasma lipoproteins, FEBS Lett 38, 247-258.). These findings offer the most detailed structural information available for the interaction between an apolipoprotein and the phospholipids of the lipoprotein surface and provide the first direct structural support for the snorkel hypothesis.

PubMed: 15209504
DOI: 10.1021/bi049817l

実験手法

SOLUTION NMR