1SNJ

Solution structure of the DNA three-way junction with the A/C-stacked conformation

1SNJ の概要

• エントリーDOI: 10.2210/pdb1snj/pdb
• 分子名称: 36-MER (1 entity in total)
• 機能のキーワード: dna, three-way junction, residual dipolar coupling, holliday junction, hammerhead
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11053.05

構造登録者

Wu, B.,Girard, F.,van Buuren, B.,Schleucher, J.,Tessari, M.,Wijmenga, S. (登録日: 2004-03-11, 公開日: 2005-04-05, 最終更新日: 2024-05-22)

主引用文献

Wu, B.,Girard, F.,van Buuren, B.,Schleucher, J.,Tessari, M.,Wijmenga, S.
Global structure of a DNA three-way junction by solution NMR: towards prediction of 3H fold.
Nucleic Acids Res., 32:3228-3239, 2004

PubMed Abstract: Three-way junctions (3H) are the simplest and most commonly occurring branched nucleic acids. They consist of three double helical arms (A to C), connected at the junction point, with or without a number of unpaired bases in one or more of the three different strands. Three-way junctions with two unpaired bases in one strand (3HS2) have a high tendency to adopt either of two alternative stacked conformations in which two of the three arms A, B and C are coaxially stacked, i.e. A/B-stacked or A/C-stacked. Empirical stacking rules, which successfully predict for DNA 3HS2 A/B-stacking preference from sequence, have been extended to A/C-stacked conformations. Three novel DNA 3HS2 sequences were designed to test the validity of these extended stacking rules and their conformational behavior was studied by solution NMR. All three show the predicted A/C-stacking preference even in the absence of multivalent cations. The stacking preference for both classes of DNA 3HS2 can thus be predicted from sequence. The high-resolution NMR solution structure for one of the stacked 3HS2 is also reported. It shows a well-defined local and global structure defined by an extensive set of classical NMR restraints and residual dipolar couplings. Analysis of its global conformation and that of other representatives of the 3H family, shows that the relative orientations of the stacked and non-stacked arms, are restricted to narrow regions of conformational space, which can be understood from geometric considerations. Together, these findings open up the possibility of full prediction of 3HS2 conformation (stacking and global fold) directly from sequence.

PubMed: 15199171
DOI: 10.1093/nar/gkh645

実験手法

SOLUTION NMR