1SMZ

Structure of Transportan in phospholipid bicellar solution

Summary for 1SMZ

• Entry DOI: 10.2210/pdb1smz/pdb
• NMR Information: BMRB: 6151
• Descriptor: Transportan in bicellar solution with [DMPC]/[DHPC]=0.33 (1 entity in total)
• Functional Keywords: transport protein
• Total number of polymer chains: 1
• Total formula weight: 2845.47

Authors

Barany-Wallje, E.,Andersson, A.,Maler, L.,Graslund, A. (deposition date: 2004-03-10, release date: 2004-03-16, Last modification date: 2024-05-22)

Primary citation

Andersson, A.
NMR solution structure and position of transportan in neutral phospholipid bicelles
Febs Lett., 567:265-269, 2004

PubMed Abstract: Transportan is a chimeric cell-penetrating peptide constructed from the peptides galanin and mastoparan, which has the ability to internalize living cells carrying a hydrophilic load. In this study, we have determined the NMR solution structure and investigated the position of transportan in neutral bicelles. The structure revealed a well-defined alpha-helix in the C-terminal mastoparan part of the peptide and a weaker tendency to form an alpha-helix in the N-terminal domain. The position of the peptide in relation to the membrane, as studied by adding paramagnetic probes, shows that the peptide lies parallel to, and in the head-group region of the membrane surface. This result is supported by amide proton secondary chemical shifts.

PubMed: 15178334
DOI: 10.1016/j.febslet.2004.04.079

Experimental method

SOLUTION NMR