1SMG
CALCIUM-BOUND E41A MUTANT OF THE N-DOMAIN OF CHICKEN TROPONIN C, NMR, 40 STRUCTURES
Summary for 1SMG
| Entry DOI | 10.2210/pdb1smg/pdb |
| Descriptor | TROPONIN C, CALCIUM ION (2 entities in total) |
| Functional Keywords | calcium-binding protein, muscle protein, troponin c |
| Biological source | Gallus gallus (chicken) |
| Total number of polymer chains | 1 |
| Total formula weight | 9966.13 |
| Authors | Gagne, S.M.,Li, M.X.,Sykes, B.D. (deposition date: 1997-02-04, release date: 1997-08-12, Last modification date: 2024-05-22) |
| Primary citation | Gagne, S.M.,Li, M.X.,Sykes, B.D. Mechanism of direct coupling between binding and induced structural change in regulatory calcium binding proteins. Biochemistry, 36:4386-4392, 1997 Cited by PubMed Abstract: The structural transition in troponin C induced by the binding of two calcium ions involves an "opening" of the structure, an event that triggers skeletal muscle contraction. We have solved the solution structure of a mutant (E41A) of the regulatory domain of skeletal troponin C wherein one bidentate ligand to the calcium in site I is missing. This structure remains "closed" upon calcium binding, indicating that the linkage between calcium binding and the induced conformational change has been broken. This provides a snapshot of skeletal troponin C between the off and on state and thereby valuable insight into the mechanism of regulation within skeletal TnC. Although several factors contribute to the triggering mechanism, the opening of the troponin C structure is ultimately dependent on one amino acid, Glu41. Insights into the structure of cardiac troponin C can also be derived from this skeletal mutant. PubMed: 9109645DOI: 10.1021/bi963076+ PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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