1SM4

Crystal Structure Analysis of the Ferredoxin-NADP+ Reductase from Paprika

「1FB3」から置き換えられました

1SM4 の概要

• エントリーDOI: 10.2210/pdb1sm4/pdb
• 分子名称: chloroplast ferredoxin-NADP+ oxidoreductase, PHOSPHATE ION, FLAVIN-ADENINE DINUCLEOTIDE, ... (4 entities in total)
• 機能のキーワード: oxidoreductase, rossmann fold
• 由来する生物種: Capsicum annuum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68617.71

構造登録者

Dorowski, A.,Hofmann, A.,Steegborn, C.,Boicu, M.,Huber, R. (登録日: 2004-03-08, 公開日: 2004-03-16, 最終更新日: 2023-08-23)

主引用文献

Dorowski, A.,Hofmann, A.,Steegborn, C.,Boicu, M.,Huber, R.
Crystal structure of paprika ferredoxin-NADP+ reductase. Implications for the electron transfer pathway.
J.Biol.Chem., 276:9253-9263, 2001

PubMed Abstract: cDNA of Capsicum annuum Yolo Wonder (paprika) has been prepared from total cellular RNA, and the complete gene encoding paprika ferredoxin-NADP(+) reductase (pFNR) precursor was sequenced and cloned from this cDNA. Fusion to a T7 promoter allowed expression in Escherichia coli. Both native and recombinant pFNR were purified to homogeneity and crystallized. The crystal structure of pFNR has been solved by Patterson search techniques using the structure of spinach ferredoxin-NADP(+) reductase as search model. The structure was refined at 2.5-A resolution to a crystallographic R-factor of 19.8% (R(free) = 26.5%). The overall structure of pFNR is similar to other members of the ferredoxin-NADP(+) reductase family, the major differences concern a long loop (residues 167-177) that forms part of the FAD binding site and some of the variable loops in surface regions. The different orientation of the FAD binding loop leads to a tighter interaction between pFNR and the adenine moiety of FAD. The physiological redox partners [2Fe-2S]-ferredoxin I and NADP(+) were modeled into the native structure of pFNR. The complexes reveal a protein-protein interaction site that is consistent with existing biochemical data and imply possible orientations for the side chain of tyrosine 362, which has to be displaced by the nicotinamide moiety of NADP(+) upon binding. A reasonable electron transfer pathway could be deduced from the modeled structures of the complexes.

PubMed: 11053431
DOI: 10.1074/jbc.M004576200

実験手法

X-RAY DIFFRACTION (2.5 Å)