1SJR

NMR Structure of RRM2 from Human Polypyrimidine Tract Binding Protein Isoform 1 (PTB1)

1SJR の概要

• エントリーDOI: 10.2210/pdb1sjr/pdb
• 関連するPDBエントリー: 1sjq
• NMR情報: BMRB: 6177
• 分子名称: Polypyrimidine tract-binding protein 1 (1 entity in total)
• 機能のキーワード: extended babbab motif, rna binding protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P26599
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17758.07

構造登録者

Simpson, P.J.,Monie, T.P.,Szendroi, A.,Davydova, N.,Tyzack, J.K.,Conte, M.R.,Read, C.M.,Cary, P.D.,Svergun, D.I.,Konarev, P.V.,Petoukhov, M.V.,Curry, S.,Matthews, S.J. (登録日: 2004-03-04, 公開日: 2004-09-14, 最終更新日: 2024-05-22)

主引用文献

Simpson, P.J.,Monie, T.P.,Szendroi, A.,Davydova, N.,Tyzack, J.K.,Conte, M.R.,Read, C.M.,Cary, P.D.,Svergun, D.I.,Konarev, P.V.,Curry, S.,Matthews, S.J.
Structure and RNA Interactions of the N-Terminal RRM Domains of PTB
Structure, 12:1631-1643, 2004

PubMed Abstract: The polypyrimidine tract binding protein (PTB) is an important regulator of alternative splicing that also affects mRNA localization, stabilization, polyadenylation, and translation. NMR structural analysis of the N-terminal half of PTB (residues 55-301) shows a canonical structure for RRM1 but reveals novel extensions to the beta strands and C terminus of RRM2 that significantly modify the beta sheet RNA binding surface. Although PTB contains four RNA recognition motifs (RRMs), it is widely held that only RRMs 3 and 4 are involved in RNA binding and that RRM2 mediates homodimerization. However, we show here not only that the RRMs 1 and 2 contribute substantially to RNA binding but also that full-length PTB is monomeric, with an elongated structure determined by X-ray solution scattering that is consistent with a linear arrangement of the constituent RRMs. These new insights into the structure and RNA binding properties of PTB suggest revised models of its mechanism of action.

PubMed: 15341728
DOI: 10.1016/j.str.2004.07.008

実験手法

SOLUTION NMR