1SJM

Nitrite bound copper containing nitrite reductase

1SJM の概要

• エントリーDOI: 10.2210/pdb1sjm/pdb
• 関連するPDBエントリー: 1J9Q
• 分子名称: Copper-containing nitrite reductase, COPPER (II) ION, NITRITE ION, ... (6 entities in total)
• 機能のキーワード: copper, nitrite, oxidoreductase
• 由来する生物種: Alcaligenes faecalis
• 細胞内の位置: Periplasm: P38501
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 111572.60

構造登録者

Tocheva, E.I.,Rosell, F.I.,Mauk, A.G.,Murphy, M.E.P. (登録日: 2004-03-03, 公開日: 2004-06-22, 最終更新日: 2023-08-23)

主引用文献

Tocheva, E.I.,Rosell, F.I.,Mauk, A.G.,Murphy, M.E.
Side-on copper-nitrosyl coordination by nitrite reductase.
Science, 304:867-870, 2004

PubMed Abstract: A copper-nitrosyl intermediate forms during the catalytic cycle of nitrite reductase, the enzyme that mediates the committed step in bacterial denitrification. The crystal structure of a type 2 copper-nitrosyl complex of nitrite reductase reveals an unprecedented side-on binding mode in which the nitrogen and oxygen atoms are nearly equidistant from the copper cofactor. Comparison of this structure with a refined nitrite-bound crystal structure explains how coordination can change between copper-oxygen and copper-nitrogen during catalysis. The side-on copper-nitrosyl in nitrite reductase expands the possibilities for nitric oxide interactions in copper proteins such as superoxide dismutase and prions.

PubMed: 15131305
DOI: 10.1126/science.1095109

実験手法

X-RAY DIFFRACTION (1.4 Å)