1SI8

Crystal structure of E. faecalis catalase

1SI8 の概要

• エントリーDOI: 10.2210/pdb1si8/pdb
• 分子名称: Catalase, SULFATE ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: n-terminal arm; anti-parallel beta-barrel; wrapping region; c-terminal helical region; tetramer; heme group, oxidoreductase
• 由来する生物種: Enterococcus faecalis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 222016.05

構造登録者

Hakansson, K.O.,Brugna, M.,Tasse, L. (登録日: 2004-02-28, 公開日: 2004-05-04, 最終更新日: 2023-08-23)

主引用文献

Hakansson, K.O.,Brugna, M.,Tasse, L.
The three-dimensional structure of catalase from Enterococcus faecalis.
Acta Crystallogr.,Sect.D, 60:1374-1380, 2004

PubMed Abstract: Enterococcus faecalis haem catalase was crystallized using lithium sulfate at neutral pH. The crystals belong to space group R3, with unit-cell parameters a = b = 236.9, c = 198.1 A. The three-dimensional structure was determined by molecular replacement using a subunit of the Proteus mirabilis catalase structure. It was refined against 2.3 A synchrotron data to a free R factor of 21.8%. Like other catalases, the E. faecalis catalase is a homotetramer with a fold and structure similar to those of its structurally closest relative P. mirabilis. The solvent structure in the active site is identical in the four subunits but differs from that found in other catalases. The structural consequences of the Ramachandran outlier Ser196 are discussed.

PubMed: 15272159
DOI: 10.1107/S0907444904012004

実験手法

X-RAY DIFFRACTION (2.3 Å)