1SHK

THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI

Summary for 1SHK

• Entry DOI: 10.2210/pdb1shk/pdb
• Descriptor: SHIKIMATE KINASE, MAGNESIUM ION (3 entities in total)
• Functional Keywords: shikimate kinase, phosphoryl transfer, adp, shikimate pathway, p-loop protein, transferase
• Biological source: Erwinia chrysanthemi
• Cellular location: Cytoplasm (By similarity): P10880
• Total number of polymer chains: 2
• Total formula weight: 38024.42

Authors

Krell, T.,Coggins, J.R.,Lapthorn, A.J. (deposition date: 1997-10-27, release date: 1998-11-18, Last modification date: 2024-10-23)

Primary citation

Krell, T.,Coyle, J.E.,Horsburgh, M.J.,Coggins, J.R.,Lapthorn, A.J.
Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi.
Acta Crystallogr.,Sect.D, 53:612-614, 1997

PubMed Abstract: Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia coli has been crystallized by the vapour-diffusion method using sodium chloride as a precipitant. Mass spectrometry was used to confirm the purity of the shikimate kinase and dynamic light scattering was used to assess conditions for the monodispersity of the enzyme. The crystals are tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 3.6 A(3) Da(-1).

PubMed: 15299895
DOI: 10.1107/S0907444997004319

Experimental method

X-RAY DIFFRACTION (1.9 Å)