1SHK

THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI

1SHK の概要

• エントリーDOI: 10.2210/pdb1shk/pdb
• 分子名称: SHIKIMATE KINASE, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: shikimate kinase, phosphoryl transfer, adp, shikimate pathway, p-loop protein, transferase
• 由来する生物種: Erwinia chrysanthemi
• 細胞内の位置: Cytoplasm (By similarity): P10880
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38024.42

構造登録者

Krell, T.,Coggins, J.R.,Lapthorn, A.J. (登録日: 1997-10-27, 公開日: 1998-11-18, 最終更新日: 2024-10-23)

主引用文献

Krell, T.,Coyle, J.E.,Horsburgh, M.J.,Coggins, J.R.,Lapthorn, A.J.
Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi.
Acta Crystallogr.,Sect.D, 53:612-614, 1997

PubMed Abstract: Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia coli has been crystallized by the vapour-diffusion method using sodium chloride as a precipitant. Mass spectrometry was used to confirm the purity of the shikimate kinase and dynamic light scattering was used to assess conditions for the monodispersity of the enzyme. The crystals are tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to contain two molecules, corresponding to a packing density of 3.6 A(3) Da(-1).

PubMed: 15299895
DOI: 10.1107/S0907444997004319

実験手法

X-RAY DIFFRACTION (1.9 Å)