1SHB

CRYSTAL STRUCTURE OF THE PHOSPHOTYROSINE RECOGNITION DOMAIN SH2 OF V-SRC COMPLEXED WITH TYROSINE-PHOSPHORYLATED PEPTIDES

Summary for 1SHB

• Entry DOI: 10.2210/pdb1shb/pdb
• Descriptor: V-SRC SH2 DOMAIN, PHOSPHOPEPTIDE B (3 entities in total)
• Functional Keywords: phosphotransferase
• Biological source: Rous sarcoma virus; More
• Total number of polymer chains: 2
• Total formula weight: 12672.21

Authors

Waksman, G.,Kuriyan, J. (deposition date: 1992-08-18, release date: 1993-10-31, Last modification date: 2024-10-16)

Primary citation

Waksman, G.,Kominos, D.,Robertson, S.C.,Pant, N.,Baltimore, D.,Birge, R.B.,Cowburn, D.,Hanafusa, H.,Mayer, B.J.,Overduin, M.,Resh, M.D.,Rios, C.B.,Silverman, L.,Kuriyan, J.
Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides.
Nature, 358:646-653, 1992

PubMed Abstract: Three-dimensional structures of complexes of the SH2 domain of the v-src oncogene product with two phosphotyrosyl peptides have been determined by X-ray crystallography at resolutions of 1.5 and 2.0 A, respectively. A central antiparallel beta-sheet in the structure is flanked by two alpha-helices, with peptide binding mediated by the sheet, intervening loops and one of the helices. The specific recognition of phosphotyrosine involves amino-aromatic interactions between lysine and arginine side chains and the ring system in addition to hydrogen-bonding interactions with the phosphate.

PubMed: 1379696
DOI: 10.1038/358646a0

Experimental method

X-RAY DIFFRACTION (2 Å)