1SHA

CRYSTAL STRUCTURE OF THE PHOSPHOTYROSINE RECOGNITION DOMAIN SH2 OF V-SRC COMPLEXED WITH TYROSINE-PHOSPHORYLATED PEPTIDES

1SHA の概要

• エントリーDOI: 10.2210/pdb1sha/pdb
• 分子名称: V-SRC SH2 DOMAIN, PHOSPHOPEPTIDE A (3 entities in total)
• 機能のキーワード: phosphotransferase
• 由来する生物種: Rous sarcoma virus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12672.25

構造登録者

Waksman, G.,Kuriyan, J. (登録日: 1992-08-18, 公開日: 1993-10-31, 最終更新日: 2024-10-30)

主引用文献

Waksman, G.,Kominos, D.,Robertson, S.C.,Pant, N.,Baltimore, D.,Birge, R.B.,Cowburn, D.,Hanafusa, H.,Mayer, B.J.,Overduin, M.,Resh, M.D.,Rios, C.B.,Silverman, L.,Kuriyan, J.
Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides.
Nature, 358:646-653, 1992

PubMed Abstract: Three-dimensional structures of complexes of the SH2 domain of the v-src oncogene product with two phosphotyrosyl peptides have been determined by X-ray crystallography at resolutions of 1.5 and 2.0 A, respectively. A central antiparallel beta-sheet in the structure is flanked by two alpha-helices, with peptide binding mediated by the sheet, intervening loops and one of the helices. The specific recognition of phosphotyrosine involves amino-aromatic interactions between lysine and arginine side chains and the ring system in addition to hydrogen-bonding interactions with the phosphate.

PubMed: 1379696
DOI: 10.1038/358646a0

実験手法

X-RAY DIFFRACTION (1.5 Å)