1SG5

Solution structure of Yaeo, a Rho-specific inhibitor of transcription termination

1SG5 の概要

• エントリーDOI: 10.2210/pdb1sg5/pdb
• 分子名称: orf, hypothetical protein (1 entity in total)
• 機能のキーワード: a+b protein, montreal-kingston bacterial structural genomics initiative, bsgi, structural genomics, transcription, protein binding
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9708.90

構造登録者

Gutierrez, P.,Gehring, K.,Montreal-Kingston Bacterial Structural Genomics Initiative (BSGI) (登録日: 2004-02-23, 公開日: 2005-07-12, 最終更新日: 2024-05-22)

主引用文献

Gutierrez, P.,Kozlov, G.,Gabrielli, L.,Elias, D.,Osborne, M.J.,Gallouzi, I.E.,Gehring, K.
Solution Structure of YaeO, a Rho-specific Inhibitor of Transcription Termination
J.Biol.Chem., 282:23348-23353, 2007

PubMed Abstract: Rho-dependent transcription termination is an essential process for the regulation of bacterial gene expression. Thus far, only two Rho-specific inhibitors of bacterial transcription termination have been described, the psu protein from the satellite bacteriophage P4 and YaeO from Escherichia coli. Here, we report the solution structure of YaeO, the first of a Rho-specific inhibitor of transcription termination. YaeO is an acidic protein composed of an N-terminal helix and a seven-stranded beta sandwich. NMR chemical shift perturbation experiments revealed that YaeO binds proximal to the primary nucleic acid binding site of Rho. Based on the NMR titrations, a docked model of the YaeO-Rho complex was calculated. These results suggest that YaeO binds outside the Rho hexamer, acting as a competitive inhibitor of RNA binding. In vitro gel shift assays confirmed the inhibition of nucleic acid binding to Rho. Site-directed mutagenesis showed that the negative character of YaeO is essential for its function in vivo.

PubMed: 17565995
DOI: 10.1074/jbc.M702010200

実験手法

SOLUTION NMR