1SER

THE 2.9 ANGSTROMS CRYSTAL STRUCTURE OF T. THERMOPHILUS SERYL-TRNA SYNTHETASE COMPLEXED WITH TRNA SER

1SER の概要

• エントリーDOI: 10.2210/pdb1ser/pdb
• 分子名称: TRNASER, PROTEIN (SERYL-TRNA SYNTHETASE (E.C.6.1.1.11)) (3 entities in total)
• 機能のキーワード: protein-t-rna complex, ligase-rna complex, ligase/rna
• 由来する生物種: Thermus thermophilus; 詳細
• 細胞内の位置: Cytoplasm : P34945
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 126144.78

構造登録者

Biou, S.,Cusack, V.,Yaremchuk, A.,Tukalo, M. (登録日: 1994-02-21, 公開日: 1994-04-30, 最終更新日: 2024-02-14)

主引用文献

Biou, V.,Yaremchuk, A.,Tukalo, M.,Cusack, S.
The 2.9 A crystal structure of T. thermophilus seryl-tRNA synthetase complexed with tRNA(Ser).
Science, 263:1404-1410, 1994

PubMed Abstract: The crystal structure of Thermus thermophilus seryl-transfer RNA synthetase, a class 2 aminoacyl-tRNA synthetase, complexed with a single tRNA(Ser) molecule was solved at 2.9 A resolution. The structure revealed how insertion of conserved base G20b from the D loop into the core of the tRNA determines the orientation of the long variable arm, which is a characteristic feature of most serine specific tRNAs. On tRNA binding, the antiparallel coiled-coil domain of one subunit of the synthetase makes contacts with the variable arm and T psi C loop of the tRNA and directs the acceptor stem of the tRNA into the active site of the other subunit. Specificity depends principally on recognition of the shape of tRNA(Ser) through backbone contacts and secondarily on sequence specific interactions.

PubMed: 8128220

実験手法

X-RAY DIFFRACTION (2.9 Å)