1SDS

Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA

1SDS の概要

• エントリーDOI: 10.2210/pdb1sds/pdb
• 分子名称: box H/ACA sRNA, 50S ribosomal protein L7Ae, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: protein-rna complex, protein-rna complex complex, protein/rna complex
• 由来する生物種: Methanocaldococcus jannaschii
• 細胞内の位置: Cytoplasm : P54066
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 53146.17

構造登録者

Hamma, T.,Ferre-D'Amare, A. (登録日: 2004-02-13, 公開日: 2004-05-18, 最終更新日: 2024-02-14)

主引用文献

Hamma, T.,Ferre-D'Amare, A.
Structure of Protein L7Ae Bound to a K-Turn Derived from an Archaeal Box H/ACA sRNA at 1.8 A Resolution.
STRUCTURE, 12:893-903, 2004

PubMed Abstract: The archaeal RNA binding protein L7Ae and its eukaryotic homolog 15.5 kDa/Snu13 recognize K-turns. This structural motif is canonically comprised of two stems (one with tandem A.G base pairs, the other with Watson-Crick pairs) linked by an asymmetric internal loop. L7Ae recognizes conventional K-turns in ribosomal and box C/D RNAs but also binds specifically to some box H/ACA RNAs at terminal stem loops. These have the A.G paired stem, but lack the Watson-Crick stem. The structure of Methanococcus jannaschii L7Ae bound to a symmetric duplex RNA without Watson-Crick stems demonstrates how a binding site for this component of diverse ribonucleoprotein complexes can be constructed with only the A.G stem and the loop. The RNA adopts a functional conformation with the aid of a base triple and tight binding of divalent cations. Comparison with the 15.5 kDa/Snu13-RNA complex structure suggests why the eukaryotic homolog does not recognize terminal stem loop L7Ae binding sites.

PubMed: 15130481
DOI: 10.1016/j.str.2004.03.015

実験手法

X-RAY DIFFRACTION (1.8 Å)