1SCL
THE SARCIN-RICIN LOOP, A MODULAR RNA
Summary for 1SCL
| Entry DOI | 10.2210/pdb1scl/pdb |
| Descriptor | RNA SARCIN-RICIN LOOP (1 entity in total) |
| Functional Keywords | rna, sarcin-ricin loop |
| Biological source | Rattus norvegicus (Norway rat) |
| Total number of polymer chains | 1 |
| Total formula weight | 9376.68 |
| Authors | Szewczak, A.A.,Moore, P.B. (deposition date: 1994-11-11, release date: 1995-01-26, Last modification date: 2024-05-22) |
| Primary citation | Szewczak, A.A.,Moore, P.B.,Chang, Y.L.,Wool, I.G. The conformation of the sarcin/ricin loop from 28S ribosomal RNA. Proc.Natl.Acad.Sci.USA, 90:9581-9585, 1993 Cited by PubMed Abstract: The sarcin/ricin loop is a highly conserved sequence found in the RNA of all large ribosomal subunits. The cytotoxins alpha-sarcin and ricin both inactivate ribosomes by cleaving a single bond in that loop. Once it has been attacked, ribosomes no longer interact with elongation factors properly, and translation stops. We have determined the conformation of the sarcin/ricin loop by multinuclear NMR spectroscopy using E73, a 29-nucleotide RNA that has the sarcin/ricin loop sequence and that is sensitive to both toxins in vitro. The sarcin/ricin loop has a compact structure that contains several purine.purine base pairs, a GAGA tetraloop, and a bulged guanosine adjacent to a reverse Hoogsteen A.U pair. It is stabilized by an unusual set of cross-strand base-stacking interactions and imino proton to phosphate oxygen hydrogen bonds. In addition to having interesting structural features, this model explains many of the biochemical observations made about the loop's structure and its reactivity with cytotoxins, and it sheds light on the loop's interactions with elongation factors. PubMed: 8415744DOI: 10.1073/pnas.90.20.9581 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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