1SCL

THE SARCIN-RICIN LOOP, A MODULAR RNA

1SCL の概要

• エントリーDOI: 10.2210/pdb1scl/pdb
• 分子名称: RNA SARCIN-RICIN LOOP (1 entity in total)
• 機能のキーワード: rna, sarcin-ricin loop
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9376.68

構造登録者

Szewczak, A.A.,Moore, P.B. (登録日: 1994-11-11, 公開日: 1995-01-26, 最終更新日: 2024-05-22)

主引用文献

Szewczak, A.A.,Moore, P.B.,Chang, Y.L.,Wool, I.G.
The conformation of the sarcin/ricin loop from 28S ribosomal RNA.
Proc.Natl.Acad.Sci.USA, 90:9581-9585, 1993

PubMed Abstract: The sarcin/ricin loop is a highly conserved sequence found in the RNA of all large ribosomal subunits. The cytotoxins alpha-sarcin and ricin both inactivate ribosomes by cleaving a single bond in that loop. Once it has been attacked, ribosomes no longer interact with elongation factors properly, and translation stops. We have determined the conformation of the sarcin/ricin loop by multinuclear NMR spectroscopy using E73, a 29-nucleotide RNA that has the sarcin/ricin loop sequence and that is sensitive to both toxins in vitro. The sarcin/ricin loop has a compact structure that contains several purine.purine base pairs, a GAGA tetraloop, and a bulged guanosine adjacent to a reverse Hoogsteen A.U pair. It is stabilized by an unusual set of cross-strand base-stacking interactions and imino proton to phosphate oxygen hydrogen bonds. In addition to having interesting structural features, this model explains many of the biochemical observations made about the loop's structure and its reactivity with cytotoxins, and it sheds light on the loop's interactions with elongation factors.

PubMed: 8415744
DOI: 10.1073/pnas.90.20.9581

実験手法

SOLUTION NMR