1SCJ

CRYSTAL STRUCTURE OF SUBTILISIN-PROPEPTIDE COMPLEX

1SCJ の概要

• エントリーDOI: 10.2210/pdb1scj/pdb
• 分子名称: SUBTILISIN E, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: hydrolase, subtilisin e - propeptide, serine protease
• 由来する生物種: Bacillus subtilis; 詳細
• 細胞内の位置: Secreted: P04189 P04189
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35808.95

構造登録者

Berman, H.M.,Jain, S.C. (登録日: 1998-04-29, 公開日: 1998-12-09, 最終更新日: 2024-05-22)

主引用文献

Jain, S.C.,Shinde, U.,Li, Y.,Inouye, M.,Berman, H.M.
The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution.
J.Mol.Biol., 284:137-144, 1998

PubMed Abstract: We report here the crystallographic structure determination of an autoprocessed (Ser221Cys)-subtilisin E-propeptide complex at 2.0 A resolution. The subtilisin domain sequence has a single substitution (Ser221Cys) which has been shown to block the maturation process prior to degradation of the propeptide domain (77 residues) that acts as an intramolecular chaperon. This mutation, however, did not prevent the enzyme from cleaving its propeptide domain with a 60-80% efficiency. The current determination is the first example of a subtilisin E-propeptide complex which has been autoprocessed. A previous structure determination of a BPN'-prosegment complex has been reported in which the subtilisin domain was extensively mutated and a calcium binding loop was deleted. Further, in this earlier determination, the complex was formed by the addition of separately expressed propeptide domain. The structure determination reported here provides additional information about the nature of the interaction between the subtilisin and propeptide domains in this complex.

PubMed: 9811547
DOI: 10.1006/jmbi.1998.2161

実験手法

X-RAY DIFFRACTION (2 Å)