1SCD

X-RAY CRYSTAL STRUCTURE OF CROSS-LINKED SUBTILISM CARLSBERG IN WATER VS. ACETONITRILE

1SCD の概要

• エントリーDOI: 10.2210/pdb1scd/pdb
• 分子名称: SUBTILISIN CARLSBERG, CALCIUM ION (3 entities in total)
• 機能のキーワード: serine protease
• 由来する生物種: Bacillus licheniformis
• 細胞内の位置: Secreted: P00780
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27386.35

構造登録者

Fitzpatrick, P.A.,Ringe, D.,Klibanov, A.M. (登録日: 1993-08-23, 公開日: 1994-01-31, 最終更新日: 2024-02-14)

主引用文献

Fitzpatrick, P.A.,Ringe, D.,Klibanov, A.M.
X-ray crystal structure of cross-linked subtilisin Carlsberg in water vs. acetonitrile.
Biochem.Biophys.Res.Commun., 198:675-681, 1994

PubMed Abstract: The crystal structure of subtilisin Carlsberg lightly cross-linked with glutaraldehyde was solved in aqueous solution by X-ray crystallography at 2.3 A resolution. It was found to be virtually identical to the recently determined (Fitzpatrick, P.A., Steinmetz, A.C.U., Ringe, D.A. & Klibanov, A.M. (1993) Proc. Natl. Acad. Sci. USA 90, 8653) structure of the cross-linked enzyme in anhydrous acetonitrile. The latter structure was found to be significantly more rigid than in water, as reflected by their average B factors. The numbers of subtilisin-bound water molecules in the two structures are similar (114 and 99 in water and in acetonitrile, respectively), but the locations of some half of these bound waters are distinct.

PubMed: 8297378
DOI: 10.1006/bbrc.1994.1098

実験手法

X-RAY DIFFRACTION (2.3 Å)