1SBS

CRYSTAL STRUCTURE OF AN ANTI-HCG FAB

Summary for 1SBS

• Entry DOI: 10.2210/pdb1sbs/pdb
• Descriptor: MONOCLONAL ANTIBODY 3A2, SULFATE ION, ... (4 entities in total)
• Functional Keywords: monoclonal antibody, fab-fragment, reproduction
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 2
• Total formula weight: 48655.00

Authors

Fotinou, C.,Beauchamp, J.,Emsley, P.,Dehaan, A.,Schielen, W.J.G.,Bos, E.,Isaacs, N.W. (deposition date: 1998-04-08, release date: 1999-04-13, Last modification date: 2024-10-30)

Primary citation

Fotinou, C.,Beauchamp, J.,Emsley, P.,deHaan, A.,Schielen, W.J.,Bos, E.,Isaacs, N.W.
Structure of an Fab fragment against a C-terminal peptide of hCG at 2.0 A resolution.
J.Biol.Chem., 273:22515-22518, 1998

PubMed Abstract: 3A2 is an antibody raised against human chorionic gonadotropin and recognizes a linear epitope on the C-terminal peptide of the human chorionic gonadotropin beta-subunit. Its three-dimensional structure has been determined to 2-A resolution using molecular replacement and refined to a conventional R-factor of 18.2%. The protein exhibits the typical immunoglobulin fold, and the model contains 944 ordered water molecules and one sulfate ion. A comparison of the complementarity-determining regions of the Fab3A2 with those from the Protein Data Bank following the canonical structure method reveals a canonical main chain conformation. This antibody belongs to the canonical structure class (combination of canonical conformations of the complementarity determining loops) that shows a preference for haptens and not for peptides. However, the shape of the surface of the antigen binding loops resembles that of an anti-peptide antibody.

PubMed: 9712877
DOI: 10.1074/jbc.273.35.22515

Experimental method

X-RAY DIFFRACTION (2 Å)