1SBP

1.7 ANGSTROMS REFINED STRUCTURE OF SULFATE-BINDING PROTEIN INVOLVED IN ACTIVE TRANSPORT AND NOVEL MODE OF SULFATE BINDING

1SBP の概要

• エントリーDOI: 10.2210/pdb1sbp/pdb
• 分子名称: SULFATE-BINDING PROTEIN, SULFATE ION (3 entities in total)
• 機能のキーワード: binding protein
• 由来する生物種: Salmonella typhimurium
• 細胞内の位置: Periplasm: P02906
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34625.59

構造登録者

Sack, J.S.,Quiocho, F.A. (登録日: 1993-07-19, 公開日: 1993-10-31, 最終更新日: 2024-02-14)

主引用文献

He, J.J.,Quiocho, F.A.
Dominant role of local dipoles in stabilizing uncompensated charges on a sulfate sequestered in a periplasmic active transport protein.
Protein Sci., 2:1643-1647, 1993

PubMed Abstract: Electrostatic interactions are among the key factors determining the structure and function of proteins. Here we report experimental results that illuminate the functional importance of local dipoles to these interactions. The refined 1.7-A X-ray structure of the liganded form of the sulfate-binding protein, a primary sulfate active transport receptor of Salmonella typhimurium, shows that the sulfate dianion is completely buried and bound by hydrogen bonds (mostly main-chain peptide NH groups) and van der Waals forces. The sulfate is also closely linked, via one of these peptide units, to a His residue. It is also adjacent to the N-termini of three alpha-helices, of which the two shortest have their C-termini "capped" by Arg residues. Site-directed mutagenesis of the recombinant Escherichia coli sulfate receptor had no effect on sulfate-binding activity when an Asn residue was substituted for the positively charged His and the two Arg (changed singly and together) residues. These results, combined with other observations, further solidify the idea that stabilization of uncompensated charges in a protein is a highly localized process that involves a collection of local dipoles, including those of peptide units confined to the first turns of helices. The contribution of helix macrodipoles appears insignificant.

PubMed: 8251939

実験手法

X-RAY DIFFRACTION (1.7 Å)