1SAN

THE DES(1-6)ANTENNAPEDIA HOMEODOMAIN: COMPARISON OF THE NMR SOLUTION STRUCTURE AND THE DNA BINDING AFFINITY WITH THE INTACT ANTENNAPEDIA HOMEODOMAIN

1SAN の概要

• エントリーDOI: 10.2210/pdb1san/pdb
• 分子名称: ANTENNAPEDIA PROTEIN (1 entity in total)
• 機能のキーワード: dna-binding protein, dna binding protein
• 由来する生物種: Drosophila melanogaster (fruit fly)
• 細胞内の位置: Nucleus (Probable): P02833
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7875.15

構造登録者

Qian, Y.Q.,Resendez-Perez, D.,Gehring, W.J.,Wuthrich, K. (登録日: 1994-01-07, 公開日: 1994-04-30, 最終更新日: 2024-05-22)

主引用文献

Qian, Y.Q.,Resendez-Perez, D.,Gehring, W.J.,Wuthrich, K.
The des(1-6)antennapedia homeodomain: comparison of the NMR solution structure and the DNA-binding affinity with the intact Antennapedia homeodomain.
Proc.Natl.Acad.Sci.USA, 91:4091-4095, 1994

PubMed Abstract: The nuclear magnetic resonance (NMR) solution structure of an N-terminally truncated mutant Antennapedia homeodomain, des(1-6)Antp(C39S), has been determined from 935 nuclear Overhauser effect upper distance constraints and 148 dihedral angle constraints by using the programs DIANA and OPAL. Twenty conformers representing the solution structure of des(1-6)Antp(C39S) have an average root-mean-square distance relative to the mean coordinates of 0.56 A for the backbone atoms of residues 8-59. Comparison with the intact Antp(C39S) homeodomain shows that the two proteins have identical molecular architectures. The removal of the N-terminal residues 1-6, which are flexibly disordered in the intact homeodomain, causes only strictly localized structure variations and does not noticeably affect the adjoining helix I from residues 10-21. The DNA-binding constant of des(1-6)Antp(C39S) is approximately 10-fold reduced relative to the intact Antp(C39S) homeodomain, which can now be attributed to the absence of the previously reported contacts of the N-terminal polypeptide segment of the intact Antp(C39S) homeodomain with the minor groove of the DNA duplex.

PubMed: 7909611
DOI: 10.1073/pnas.91.9.4091

実験手法

SOLUTION NMR