1S7A

NMR structure of the La motif of human La protein

1S7A の概要

• エントリーDOI: 10.2210/pdb1s7a/pdb
• 関連するPDBエントリー: 1OWX 1S79
• NMR情報: BMRB: 6044
• 分子名称: Lupus La protein (1 entity in total)
• 機能のキーワード: la motif, alpha/beta, winged helix domain, rna binding protein, translation
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus (Probable): P05455
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12020.79

構造登録者

Alfano, C.,Sanfelice, D.,Babon, J.,Kelly, G.,Jacks, A.,Curry, S.,Conte, M.R. (登録日: 2004-01-29, 公開日: 2004-04-06, 最終更新日: 2024-05-22)

主引用文献

Alfano, C.,Sanfelice, D.,Babon, J.,Kelly, G.,Jacks, A.,Curry, S.,Conte, M.R.
Structural analysis of cooperative RNA binding by the La motif and central RRM domain of human La protein.
Nat.Struct.Mol.Biol., 11:323-329, 2004

PubMed Abstract: The La protein is a conserved component of eukaryotic ribonucleoprotein complexes that binds the 3' poly(U)-rich elements of nascent RNA polymerase III (pol III) transcripts to assist folding and maturation. This specific recognition is mediated by the N-terminal domain (NTD) of La, which comprises a La motif and an RNA recognition motif (RRM). We have determined the solution structures of both domains and show that the La motif adopts an alpha/beta fold that comprises a winged-helix motif elaborated by the insertion of three helices. Chemical shift mapping experiments show that these insertions are involved in RNA interactions. They further delineate a distinct surface patch on each domain-containing both basic and aromatic residues-that interacts with RNA and accounts for the cooperative binding of short oligonucleotides exhibited by the La NTD.

PubMed: 15004549
DOI: 10.1038/nsmb747

実験手法

SOLUTION NMR